dna sequence and betaine

Chris LaRosa clarosa at biocomp.unl.edu
Mon Jul 10 13:48:25 EST 2000



1 M betaine can relax the gc rich areas.... It should be used in the pcr
reactions used to generate templates, and reportedly is useful in
finishing projects for recovering sequence from secondary structure and
gc rich regions.  Other tricks.... using different chemistries, dna
polymerases , machines..   Certain taqs are poor for finishing i.e.
amplitaq DNA polymerase FS and the ABI prism chemistries (with most
market share) is poor for homopolymer regions, poly A., gc rich.  Thats
why ABI came up with formulations to work around the problem reagions.






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