how to make nuclease free water ?

Axel Schwekendiek schwekdk at
Tue Jul 11 10:26:43 EST 2000

Dear all,

could anybody provide me with a short protocol on how to prepare nuclease
free water? I need it for PCR, RT-PCR and RNA extraction from plant tissues.
We have a milli-Q system and I don't want to use DEPC.

Any hints would be very much appreciated,


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