how to make nuclease free water ?

Nick Theodorakis nicholas_theodorakis at
Tue Jul 11 10:38:12 EST 2000

"Axel Schwekendiek" <schwekdk at> wrote:
>Dear all,
>could anybody provide me with a short protocol on how to
prepare nuclease
>free water? I need it for PCR, RT-PCR and RNA extraction from
plant tissues.
>We have a milli-Q system and I don't want to use DEPC.
>Any hints would be very much appreciated,

Water from a well-maintained Milli-Q system should already be
nuclease-free without the need further treatment.

Nick Theodorakis

nicholas_theodorakis at


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