how to make nuclease free water ?

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Tue Jul 11 10:38:12 EST 2000


"Axel Schwekendiek" <schwekdk at uni-hohenheim.de> wrote:
>Dear all,
>
>could anybody provide me with a short protocol on how to
prepare nuclease
>free water? I need it for PCR, RT-PCR and RNA extraction from
plant tissues.
>We have a milli-Q system and I don't want to use DEPC.
>
>Any hints would be very much appreciated,
>
>Axel
>

Water from a well-maintained Milli-Q system should already be
nuclease-free without the need further treatment.


Nick Theodorakis

nicholas_theodorakis at urmc.rochester.edu

-----------------------------------------------------------

Got questions?  Get answers over the phone at Keen.com.
Up to 100 minutes free!
http://www.keen.com






More information about the Methods mailing list