Coomassie Blue: destaining

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Fri Jul 14 05:41:17 EST 2000

This message has been posted by:  "Luis M. Martins" <L.Martins at>


I am using PhastGel BlueR coomassie blue dye from Pharmacia, I am concearned
with destaining the gel without losing the signal from the bands (I have
around 10ng protein per band)

I am currently destaining in 10% Acetic acid, this takes quite a long time
and leaves an uneven blue background. I was considering using methanol to
improve solubility of the CB stain. How much methanol should I use without
risking loosing signal from the bands?

Your help is much appreciated!



Dr. Luis Miguel Martins
Signal Transduction Laboratory
Imperial Cancer Research Fund
44 Lincoln's Inn Fields
London WC2A 3PX

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