Coomassie Blue: destaining
jmollerup at mcb.molbio.ku.dk
Fri Jul 14 09:24:54 EST 2000
Ethanol can be used in stead of methanol when destaining, in the same
amounts as methanol usually is used. Also it can replace methanol in Western
blots - I and several others have used ethanol for years for this purpose,
without loss of resolution, immunogenicity etc.
Ph.D. Jens Mollerup
Jan Aelen <janal at nmr.kun.nl> wrote in message
news:396F0540.67E8FDE5 at nmr.kun.nl...
> For improvement 10% acetic acid and 25 % methanol.
> For a faster destaining I use a microwave-oven or a rubber of foam in a
> There is no loss of resolution.
> nospam at our.site wrote:
> > This message has been posted by: "Luis M. Martins"
<L.Martins at REMOVE-THIS-TO-SENDicrf.icnet.uk>
> > Hi
> > I am using PhastGel BlueR coomassie blue dye from Pharmacia, I am
> > with destaining the gel without losing the signal from the bands (I have
> > around 10ng protein per band)
> > I am currently destaining in 10% Acetic acid, this takes quite a long
> > and leaves an uneven blue background. I was considering using methanol
> > improve solubility of the CB stain. How much methanol should I use
> > risking loosing signal from the bands?
> > Your help is much appreciated!
> > Regards,
> > Miguel
> > --
> > Dr. Luis Miguel Martins
> > Signal Transduction Laboratory
> > Imperial Cancer Research Fund
> > 44 Lincoln's Inn Fields
> > London WC2A 3PX
> > UK
> J.M.A. Aelen
> e-mail: Janal at nmr.kun.nl
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