Coomassie Blue: destaining

mp candirectory at yahoo.com
Fri Jul 14 23:24:32 EST 2000


Hi
A very fast method is destaining in water in Microwave fro about 15 minutes, and the rest with
methanol+acetic acid

nospam at our.site wrote:

> This message has been posted by:  "Luis M. Martins" <L.Martins at REMOVE-THIS-TO-SENDicrf.icnet.uk>
>
> Hi
>
> I am using PhastGel BlueR coomassie blue dye from Pharmacia, I am concearned
> with destaining the gel without losing the signal from the bands (I have
> around 10ng protein per band)
>
> I am currently destaining in 10% Acetic acid, this takes quite a long time
> and leaves an uneven blue background. I was considering using methanol to
> improve solubility of the CB stain. How much methanol should I use without
> risking loosing signal from the bands?
>
> Your help is much appreciated!
>
> Regards,
>
> Miguel
>
> --
> Dr. Luis Miguel Martins
> Signal Transduction Laboratory
> Imperial Cancer Research Fund
> 44 Lincoln's Inn Fields
> London WC2A 3PX
> UK






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