Taq inactivation

Martin Chan s987517 at mailserv.cuhk.edu.hk
Sun Jul 16 04:04:24 EST 2000


Dear netters,
    I am going to directly use the PCR mixture for restriction digestion,
but I fear the remaining Taq in the PCR mixture will fill in the 5' overhang
generated by restriction endonucleases. Is my worry necessary? Can Taq be
inactivated by heating at 94-96C for 30 min prior to restriction digestion?
I am frustrated by the low recovery of my lab's PCR purification kit, so I
plan to skip the purification step.

Best wishes,
Martin.







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