Coomassie Blue: destaining

Peter Pediaditakis pxpst2 at dlt.pitt.edu
Sun Jul 16 10:17:44 EST 2000


In article <WatwOe77CJLh65c8OJ0=hsgAfDP3 at 4ax.com>, Allan Stensballe
<allan at NUKESPAMMERSstensballe.com> wrote:

> For visualization of ng levels of protein you should go for silver
> staining instead of CB or Sypro dyes (assumed that you stain
> electrophoretic gels and just need visualization).    
> Silver staining with the right shortcuts is nearly as fast as CB, but
> have sub ng sensitivity.

Draw back of Silver is that you can not sequence the protein......but then
again if one is looking at sequencing 10 ng of protein then they must be
working with a god on the MS MS sequencer.

-- 
Peter Pediaditakis
University of Pittsburgh
Dept. of Pathology

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