clarosa at biocomp.unl.edu
Mon Jul 17 09:49:48 EST 2000
You should inactivate taq by using one of several methods. Phenol
chloroform extraction followed by ethanol ppt, or one of the pcr
purification products. The kits from Qiagen, Gibco BRL, Promega,
GeneClean II or homemade glass milk kits work. Taq is plenty active at
room temp and heating is ineffective as a method of inactivation and
will fill in your restriction ends.
Someone else advocated pcr cloning the product, then cutting it out as a
solution for problem ends,,,,...
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