CNBr cleavage + iodoacetamide ?
peter.cherepanov at uz.kuleuven.ac.be
Mon Jul 17 11:35:38 EST 2000
does somebody know for sure:
if CNBr would cleave at Cysteines when the protein was treated with
(logically, the reaction proceeds through a 5-member ring during cleavage
at Met. With alkylated Cys there would be a 4-member ring, which should not
favor this reaction. But on the other hand, I heard of a modification at
Cys that will make CNBr cleave at Cys+Met !!).
hope to get your advice,
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