5' RACE, how far can we go???
clarosa at biocomp.unl.edu
Tue Jul 18 16:34:32 EST 2000
The question is a bit broad. Hopefully these pointers are helpful.
There is a glut of literature you can find just by doing a google.com
starters. . Also visit the web sites of , clonetech, Qiagen,
etc. they have extensive manuals and technical bulletins. 3kb is a not
too too large, I just did a 2.4 kb rt pcr yesterday. I used to like
the clonetech Marathon kit, but I have heard others state that the gibco
reagents are better and cheaper.
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