5' RACE, how far can we go???

Chris LaRosa clarosa at biocomp.unl.edu
Tue Jul 18 16:34:32 EST 2000


The question is a bit broad.    Hopefully these pointers are helpful.
There is a glut of literature you can find just by doing a google.com
search.   http://www.nwfsc.noaa.gov/protocols/revtrpcr.html
http://biology.neehow.org/wonderful/protocols/rnaprot97.html#rt for
starters. . Also visit the web sites of , clonetech, Qiagen,
lifetech.com, stratagene,
etc. they have extensive manuals and technical bulletins.  3kb is a not
too too large, I just did a 2.4 kb rt pcr yesterday.   I used to like
the clonetech Marathon kit, but I have heard others state that the gibco
reagents are better and cheaper.






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