SDS solutions behaving, um, oddly.....
kresten at my-deja.com
Wed Jul 19 08:26:47 EST 2000
In article <8l2pnk$iee$1 at nnrp1.deja.com>,
sam_ross at my-deja.com wrote:
> Dear Netter Nutters,
> Has anybody else ever had a dilute reagent solution precipitate out,
> whilst a more concentrated one stays dissolved?
Whenever I precipitate protein by adding 1/10 vol of my 100% TCA / 0.2%
deoxycholic acid I see this.
> Anybody have any ideas
> about why this might be happening?
However, is there not something about SDS precipitating with either Na+
or K+? This difference matters when one does minipreps, since there is
a difference between using K+ acetate and Na+ acetate.
> Sam Ross
> DSTO Australia
> Sent via Deja.com http://www.deja.com/
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Kresten Lindorff Larsen
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