SDS solutions behaving, um, oddly.....

Kresten kresten at my-deja.com
Wed Jul 19 08:26:47 EST 2000


In article <8l2pnk$iee$1 at nnrp1.deja.com>,
  sam_ross at my-deja.com wrote:
> Dear Netter Nutters,
>
[snip]
>
> Has anybody else ever had a dilute reagent solution precipitate out,
> whilst a more concentrated one stays dissolved?

Whenever I precipitate protein by adding 1/10 vol of my 100% TCA / 0.2%
deoxycholic acid I see this.

> Anybody have any ideas
> about why this might be happening?

No.
However, is there not something about SDS precipitating with either Na+
or K+? This difference matters when one does minipreps, since there is
a difference between using K+ acetate and Na+ acetate.

Kresten

>
> Sam Ross
> DSTO Australia
>
> Sent via Deja.com http://www.deja.com/
> Before you buy.
>

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Kresten Lindorff Larsen


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