potato RT-PCR?

Allison B. MacKay m_ackaya_b at biology.queensu.ca
Thu Jul 20 14:31:18 EST 2000


I'm doing RT-PCR on potato tissues (leaf, stem, root,
tuber) right now, and I'm looking for a good positive
control gene.  This is to amplify to ensure that the
RT rxn is working - it should be constitutive in all
the tissues and the gene should contain introns (ie.
so I can distinguish between products amplified from
mRNA or genomic DNA).  Can anybody here advise me on
this?

-- 
Allison Mackay
Department of Biology
Queen's University
Kingston, ON






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