still waiting for suggestions please....

Supan ssupan at
Fri Jul 21 16:42:49 EST 2000

Hi! Everybody: 
I am new to this newsgroup. I am interested to know about buffers used to isolate total proteins 
from leaves of plant (i work with soybean and maize plants)? I am interested to measure the 
activity of following enzymes using the total protein (crude), i.e. Phenyl ammonia lyas, catalase, 
chitinase, glucanase, peroxidase. Anybody know a single extraction buffer to isolate total protein 
and check the enzyme activity. Furthermore, could someone please let me know whether it is 
feasable or not if i collect the leaves and gound them in liquid nitrogen and then free the powder 
at -80 C and then take the powder and dissolved in different buffer for differ enzymes? Do i need 
to freez dry the powder after crushing the leaves in liquid nitrogen or its ok if i just keep it at 
-80? If i use 80% ammonium sulfate, will i lose protein too? Any suggestion regarding total 
protein isolation will be greatly appreciated. Kindly send your suggustions to me at 
mkhan8 at
Wajahat Khan 
Plant Science Dept., 
McGill University 

mkhan8 at
mwkhan at 


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