tell me how to unstand this EST display
clarosa at biocomp.unl.edu
Sat Jul 22 10:59:37 EST 2000
sdyang at china.com wrote:
> I have search this EST from webpage of bioinfo.okstate.edu ,It will be useful to me .But there are some scientific items I can not know its meaning ,such as score,expect,positives .I need your kind help .Furthmore ,I hope you can tell how to use EST database to identify a new cDNA .you may refer me some useful articles which have explain EST more detailed .
The E values indicate the statistical significance that the matches are
not due to chance. The smaller the E value the better the match. Your
E values indicate that the probability that your match was due to chance
was below 1 x E-21.... very low. So you certainly matched very well. I
would suggest you can identify your cdna is a mitochodrial phosphate
transporter,,, that kind of amino acid matching indicates vertual
Chris, the grouch, larosa.
More information about the Methods