Weird Northern again!!!

news.mad.ttd.net jmartinez at cib.csic.es
Sun Jul 23 16:39:08 EST 2000


Chunxin Wang <cxwang at wam.umd.edu> escribió en el mensaje de noticias
Pine.GSO.4.21.0007231634430.27417-100000 at rac1.wam.umd.edu...
>
> This time two new probes PCR-amplified from arabidopsis PR-1 and VSP1
> genes, repectively, were used and the membrane was newly transferred. I
> did the hybridization at the same time in different bags. However, the
> result was the same: all signals came from the rRNAs. The hybridization
> pattern was exactly the same as the EB-stained gel pattern.
>
> why it happened to me so often in so short time?I was so desperate now.
>
> Black
>

I don´t know the previous messages but, which hybridization conditions are
you using?.

Jose Luis

--
Jose Luis Martinez Ph. D.
Departamento de Biologia Celular y Desarrollo
Centro de Investigaciones Biologicas
C/ Velazquez 144
Madrid 28006
SPAIN







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