cDNA library screening with antibody

Warren Gallin wgallin at gpu.srv.ualberta.ca
Sun Jul 23 17:17:28 EST 2000


Tony,
	The problem with that approach is that the cells used for immunization
lyse and you get an immune response to intracellular proteins as well.
	So there is no reason not to screen a library like this, your positives
will not be biased toward membrane proteins much.



Tony Schountz wrote:
> 
> Can one use antibodies generated against whole cells to screen cDNA
> expression libraries (lambda) for transmembrane proteins? Or does the TM
> domain cause problems (e.g. ppt, screwed up folding, etc.)? I would
> appreciate experienced opinions on this.
> 
> Thanks,
> 
> Tony
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