cDNA library screening with antibody

Warren Gallin wgallin at
Sun Jul 23 17:17:28 EST 2000

	The problem with that approach is that the cells used for immunization
lyse and you get an immune response to intracellular proteins as well.
	So there is no reason not to screen a library like this, your positives
will not be biased toward membrane proteins much.

Tony Schountz wrote:
> Can one use antibodies generated against whole cells to screen cDNA
> expression libraries (lambda) for transmembrane proteins? Or does the TM
> domain cause problems (e.g. ppt, screwed up folding, etc.)? I would
> appreciate experienced opinions on this.
> Thanks,
> Tony
-------------- next part --------------
A non-text attachment was scrubbed...
Name: wgallin.vcf
Type: text/x-vcard
Size: 426 bytes
Desc: Card for Warren Gallin
Url :

More information about the Methods mailing list