Rsa digestion.

Julia Liachenko jul2 at
Mon Jul 24 06:40:07 EST 2000

I am genotyping the mice for defective leptin receptor allele (db).
According to the protocol I have to run Rsa digestion on the PCR
in order to digest 135 bp fragments into 108 and 25 bp pieces.
It seems to work for heterozygotes (I get two bands), but the
reaction fails for homozygotes (I get 135 bp band instead of 108 bp).
Does anybody have any suggestions?
Your help will be appreciated.

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