Robert A. Schoenfeld
raschoenfeld at ucdavis.edu
Mon Jul 24 19:16:37 EST 2000
I have a question about quantitating northerns on a phosphoimager.
I've been blotting total RNA, probing w/a 32P labeled transcript and
scanning into a phosphoimager. After quantitating, I find myself
wondering about some of the numbers that the software spit out.
Should I expect the signal I get from 5 ug of total RNA to be half
the signal I'd get from 10 ug? Empirically, I'm not seeing this and
have been wondering why, or, like any other scientist, "what did I do
Thanks in advance,
Robert A. Schoenfeld, Ph.D.
MCB, rm. 24 Briggs Hall
Davis, CA 95616
email: raschoenfeld at ucdavis.edu
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