Re-amplification of PCR product

alex dobrovic adobrovic at medicine.adelaide.edu.au
Tue Jul 25 02:34:31 EST 2000


This is very common. You need to use a 1/10 or 1/100  or even 1/1000
dilution of your first round PCR product.


>HI, I try to re-amplify the product with the same first round pcr primers.
>Although I purified the first round pcr product with different method.
>Instead ofobtaining strong amplfied band I get weak band with lots of
>non-specific bands
>
>Thanks
>
>mike

Regards

Alex


alexander.dobrovic at adelaide.edu.au



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