Taq inactivation

A.F. Simpson AFS7 at le.ac.uk
Tue Jul 25 11:48:36 EST 2000


> Martin Chan wrote:

> > I am frustrated by the low recovery of my lab's PCR purification kit, so I
> > plan to skip the purification step.

Have you tried a simple phenol/cholorform extraction and ethanol
precipitation rather than using the purification kit?  Then you won't
have to worry about residual Taq or about buffer compatability.

love
Anna






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