Taq inactivation

Dr. Duncan Clark drc at nospam.demon.co.uk
Tue Jul 25 07:09:06 EST 2000

In article <397DC4E4.3259 at le.ac.uk>, A.F. Simpson <AFS7 at le.ac.uk> writes
>Have you tried a simple phenol/cholorform extraction and ethanol
>precipitation rather than using the purification kit?  Then you won't
>have to worry about residual Taq or about buffer compatability.

There has been another thread running on this and basically
phenol/cholorform extraction and ethanol precipitation will not fully
remove all the Taq.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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