Taq inactivation

Dr. Duncan Clark drc at nospam.demon.co.uk
Tue Jul 25 07:09:06 EST 2000


In article <397DC4E4.3259 at le.ac.uk>, A.F. Simpson <AFS7 at le.ac.uk> writes
>Have you tried a simple phenol/cholorform extraction and ethanol
>precipitation rather than using the purification kit?  Then you won't
>have to worry about residual Taq or about buffer compatability.

There has been another thread running on this and basically
phenol/cholorform extraction and ethanol precipitation will not fully
remove all the Taq.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
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