minimum time for drop dialysis?
converrl at hotmail.com
Tue Jul 25 21:46:15 EST 2000
I had a simple question:
What is the minimum amount of time for filter dialysis of a restriction
enzyme digestion in order to resuspend it in ligation buffer for an
I know that for electroporation, a 25ul rxn is good to go for zapping in 15
min. against 5ml of TE--is this long enough for ligations to work well?
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