minimum time for drop dialysis?

Richard Converse converrl at hotmail.com
Tue Jul 25 21:46:15 EST 2000


Dear archive:

   I had a simple question:

What is the minimum amount of time for filter dialysis of a restriction 
enzyme digestion in order to resuspend it in ligation buffer for an 
overnight ligation?

I know that for electroporation, a 25ul rxn is good to go for zapping in 15 
min. against 5ml of TE--is this long enough for ligations to work well?

  e-mail any responses to converrl at hotmail.com...

                 Many thanks,

                 Richard--
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