plant rna extraction : purity problems

Chris LaRosa clarosa at biocomp.unl.edu
Thu Jul 27 09:12:13 EST 2000



"Bernard Murray, PhD" wrote:
> 
> In article <397D7773.98F9923B at gengenp.rug.ac.be>, Sam Lievens
> <salie at gengenp.rug.ac.be> wrote:
> 
> > i am growing the roots in liquid medium so there's no dirt around the
> > roots at all.
> 
> I'm no plant person so I've never actually used the protocol but
> in the past a question to this newsgroup about such nucleic acid
> extractions inevitably led to the suggestion to use the CTAB method.
> Have you tried that one? (maybe it only works for DNA)
> 
>    

Perhaps incorporation of an agent that binds phenolics,   like PVPP..>?
might get rid of them, but I have no idea what it would do to rna.  It
is used in enzyme assays.






More information about the Methods mailing list