Unknow Band from PCR product
Gys de Jongh
GysdeJongh at planet.nl
Thu Jul 27 12:49:07 EST 2000
"biobiobio_2000" <biobiobio_2000 at yahoo.com> wrote in message
news:8lmrrt$ncv$1 at justice.csc.cuhk.edu.hk...
> I have checked the problem is due to loading dye. I made up my loading dye
> with Ficoll 400 and xylene cyanol and bromophenol blue. But what's the
> problem then? Will it because of Ficoll 400.
sure it was bromophenol blue ? Methylene blue is positive , will form a large
positive complex with DNA , migrates a little bit "the wrong way" (?)
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