Plasmid question

BC who at nowhere.com
Thu Jul 27 21:28:24 EST 2000


Supercoiling will cause the ligated plasmid to move faster than the linear
piece of DNA.  This will be the case for all closed circular DNA molecules.
If your looking to size the insert, cut it our or at least linearized the
vector at a unique site outside of your insert.

Hope this is useful

Brad
anthesia at iastate.edu

Mark Garry Petten wrote in message ...
>I am running a sample of plasmid I have ligated with my cDNA insert
>through a 1% TBE gel. I am also running with it a sample of linearized
>plasmid without the insert. My question is will supercoiling cause the
>ligated plamid to migrate less through the gel or more?
>
>
>*************************************************************************
>*Mark G. Petten                      * Research is the process of going *
>*Dept. of Biology                    * up alley's to see if they're     *
>*Honours                             * blind.                           *
>*Memorial University of Newfoundland * - Bates's Law of Research        *
>*************************************************************************
>







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