Protocol for making competent E coli

Susanne Rohrer "srohrer(removethis)" at
Fri Jul 28 04:15:42 EST 2000

You could have found these by yourself doing a simple medline search.

quick and dirty, and not that efficient:
One-step preparation of competent Escherichia coli: transformation and
storage of bacterial cells in the same solution. Chung et al, PNAS 1989.

Then there is always the Inoue method. Inoue et al, Gene 1990 High
efficiency transformation of Escherichia coli with plasmids

Susanne Rohrer
Institute of Medical Microbiology
University of Zurich

srohrer at immv dot unizh dot ch

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