Plasmid question

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Fri Jul 28 04:40:14 EST 2000


In article <Pine.OSF.3.95.1000727141957.2981B-100000 at plato.ucs.mun.ca>,
Mark Garry Petten <t67mgp at morgan.ucs.mun.ca> writes
>I am running a sample of plasmid I have ligated with my cDNA insert
>through a 1% TBE gel. I am also running with it a sample of linearized
>plasmid without the insert. My question is will supercoiling cause the
>ligated plamid to migrate less through the gel or more?

Don't forget there are differences in migration of various forms
depending upon whether the gel is run with EtBr or not.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk






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