Internal standard to quantify gene
clarosa at biocomp.unl.edu
Fri Jul 28 09:05:20 EST 2000
David Humair wrote:
> Hi netters,
> I have to quantify a mRNA in different potatoe clones...I want to use an
> internal constitutive standard (Rubisco??) Do you have any suggestions.
> Thanks a lot
I would be leary of using rubisco...Isnt its expression light variable
and just too variable ?? What about actin or a ribosomal gene or
ubiqutin. I used 16s ribosomal insert to normalize northerns and
everyone uses the gel pictures to claim even loading on a northern gel.
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