Separation of specific DNA fragment
Austin P. So (Hae-Jin)
haejin at netinfo.ubc.caX
Fri Jul 28 16:35:33 EST 2000
What do you mean "in vivo"?
Do you mean as an antisense molecule?
> Nope. The melting temperature (i.e. affinity) is too high. This is
> why they can be used in PCR where the temperature is raised 80+
> degrees. Other oligos will melt from their complement at these temps,
> provided they're not exonucleased or "bumped off" by the enzyme.
> The question is......can they do the same thing in vivo????
Austin P. So (Hae Jin)
University of British Columbia
E-mail: haejin at netinfo.ubc.ca
http://www.interchange.ubc.ca/haejin/index.html (under construction)
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