Separation of specific DNA fragment

Austin P. So (Hae-Jin) haejin at netinfo.ubc.caX
Fri Jul 28 16:35:33 EST 2000

What do you mean "in vivo"?

Do you mean as an antisense molecule?

GradStudent wrote:

> Nope.  The melting temperature (i.e. affinity) is too high.  This is
> why they can be used in PCR where the temperature is raised 80+
> degrees.  Other oligos will melt from their complement at these temps,
> provided they're not exonucleased or "bumped off" by the enzyme.
> The question is......can they do the same thing in vivo????

Austin P. So (Hae Jin)

Biotechnology Laboratory
University of British Columbia

E-mail: haejin at (under construction)

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