Long fragments of DNA and XL1 blue strain

Martin Offterdinger martin.offterdinger at akh-wien.ac.at
Mon Jul 31 01:39:10 EST 2000


Amelia Sanchez-Capelo <rmgzasc at ucl.ac.uk> schrieb in im Newsbeitrag:
Pine.SOL.3.96.1000721113007.21484A-100000 at socrates-a.ucl.ac.uk...
>
> Do you have experience with long frangments of DNA (>10Kb) and
electroporation
> with XL1 blue  strain? I have notice that some extrange rearragements can
> happen. I have work a lot with shorter fragments (1-3Kb) and it is
perfect.
> Could you suggest me a strain for such a long  fragments?
>
> Thank you
>
> Amelia Sanchez Capelo, PhD
> Wolfson Institute for Biomedical Research
> University College London
> Gower Street
> London, WC1E 6BT
> United Kingdom
>
> E-mail: a.sanchez-capelo at ucl.ac.uk
> -----------------------------------------
>
>
>
> ---

Stratagene (no affiliation) recommends to use the XL10gold strain for long
fragments; I used it and got nice transformation efficiencies and no
rearrangements, but plasmid yield was rather low after Maxi-prep.
martin







More information about the Methods mailing list