Temperature activity range for Taq-Ligase

Jesper jsp at imsb.au.dk
Fri Jun 2 07:58:28 EST 2000


I am thinking about using Taq ligase in a cycling ligation but I was
wondering wether it is active below 45 degrees celcius. I would like to
use a denaturations step at 95 degrees then annealing at 20-30 degrees
and ligation.... Is this possible with Taq ligase or is it only active
at elevated temperatures (at the 45-65 stated in the New England Biolabs
catalog).??

Hope to get a qualified answer soon.

- Jesper.


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