hzhen at freeuk.com
Sat Jun 3 09:40:19 EST 2000
asanchez at cibnor.mx wrote:
> Today I was talking about mercaptoethanol, and I said that it
> denaturalizes proteins. Somebody call my attention to say that it is
> not true, that mercaptoethanol is a reducer agent, not a
> protein "denaturalizer".
> Can someone tell me if I am right or not? By reducing the environment
> of the protein it denatures the protein, so it must be considered as a
> denaturing agent...or not????
Not. Reducing agent and denaturant are something completely
different. While some proteins are unstable in reducing
environment, most proteins are quite happy in a reducing
environment. Denaturant actively denatures the protein,
reducing agent doesn't. Reducing agents only affect a small
subset of proteins in which disulphide bond is important for
stability, while denaturants have a more general
> Sent via Deja.com http://www.deja.com/
> Before you buy.
More information about the Methods