DTNB alternative

Johannes Graumann j_graumann at hotmail.com
Sat Jun 3 18:05:07 EST 2000


Hi there,
I'm running into trouble determining the thiol status of my protein under
oxidizing conditions (120 myM H2O2) - DTNB (Ellman's reagent) doesn't
work here (why?).
Does anybody know of an alternative reagent that may do the job under
these conditions? I'd prefer a spectroscopic method to AMS trapping.

Thanks for any help, Johannes


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