Adenoviral vector and GFP

Masoni Marco m.masoni at dfc.unifi.it
Mon Jun 5 08:21:07 EST 2000


hi all,
is there anybody who can confirm if Green Fluorescent Protein is helpful

in screening homologous recombination in adenoviral vector cloning? If I

co-transfect 293 cells with a shuttle vector expressing a gene of
interest
together with GFP, could I use fluorescence to screen the plaques?

Any response is appreciated.

Many thanks
Marco Foschi









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