About mercaptoethanol

nospam at our.site nospam at our.site
Mon Jun 5 08:34:31 EST 2000


This message has been posted by:  612 right <i.hinners at REMOVE-THIS-TO-SENDicrf.icnet.uk>

I just want to add that cytosolic proteins are recuced and luminal and
extracellular proteins are usually oxidised, i.e. have disulfid bonds as
opposed to SH groups

ChenHA wrote:

> asanchez at cibnor.mx wrote:
> >
> > Today I was talking about mercaptoethanol, and I said that it
> > denaturalizes proteins. Somebody call my attention to say that it is
> > not true, that mercaptoethanol is a reducer agent, not a
> > protein "denaturalizer".
> >
> > Can someone tell me if I am right or not? By reducing the environment
> > of the protein it denatures the protein, so it must be considered as a
> > denaturing agent...or not????
>
> Not.  Reducing agent and denaturant are something completely
> different.  While some proteins are unstable in reducing
> environment, most proteins are quite happy in a reducing
> environment.  Denaturant actively denatures the protein,
> reducing agent doesn't.  Reducing agents only affect a small
> subset of proteins in which disulphide bond is important for
> stability, while denaturants have a more general
> applicability.
>
> > Sent via Deja.com http://www.deja.com/
> > Before you buy.

--
Ina Hinners
ICRF
Secretory Pathways Laboratory
44 Lincolns Inn Fields
WC2A 3PX London
UK
email: I.Hinners at icrf.icnet.uk







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