DNA extraction from very tough microorganism

Roland Hübner rhubner at gins.uia.ac.be
Mon Jun 5 16:27:12 EST 2000


>This bug resists heat, ultrasounds, lysozyme, proteinase K, SDS, 
>phenol. I just could not break the cells and release the DNA.

Hmmm, did you try HOT (60°C) phenol?
What about benzyl chloride or maybe GITC/urea blends at highest 
molarities?

Good luck!
 Roland






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