DNA extraction from very tough microorganism

[Susanne Rohrer]

Damien Marsic wrote:

> Commercial kits from Promega and Qiagen (using their protocol for
> gram-positive bacteria) have failed. This bug resists heat, ultrasounds,
> lysozyme, proteinase K, SDS, phenol. I just could not break the cells and
> release the DNA.

Have you got access to a bead-beater? we have a reciprocating shaker from
Bio101 that works with small glass beads - works great for Gram positives.
--
Susanne Rohrer
Institute of Medical Microbiology
University of Zurich
Switzerland

srohrer at immv dot unizh dot ch