HPLC problem

Aaron Bell bellaaro at pitt.edu
Tue Jun 6 06:39:17 EST 2000

In article <050620001536469150%nospam at nowhere.com>, Richard
<nospam at nowhere.com> wrote:

> Using fluorescence detection we get an OK baseline using 50/50
> water/methanol but as soon as we change to 45/55 acetonitrile/water the
> baseline goes crazy, even after 24 hrs equilibrium time.

It sounds like you have air bubbles in the system.  Do you keep the ACN
sparged?  Is the ACN/H2O made up as a neat solution or is it mixed by your

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