Error Prone PCR
namy at infobiogen.fr
Tue Jun 6 14:08:56 EST 2000
I have a protocole to do error prone PCR which works very well. I have been
randomly mutagenised a 2Kb gene with this protocol (the number of mutations
is beetwen one and five per fragment)
I used :
2,5 Units of taq polymerase
10uM each oligonucleotides.
(Becarfull the choice of buffer is very very important, in my hand onle CETUS
(from Perkin Elme) buffer works.
the elongation time is lowest in these conditions approxymatively 1.5min per
If you want more explanations, you can email me to namy at infobiogen.fr
More information about the Methods