Phosphorylation specific antibodies

sourav ghosh sghosh at utmem1.utmem.edu
Wed Jun 7 14:10:40 EST 2000


In article <39244C88.2FEB845C at ucd.ie>, Ruth.McMahon at ucd.ie (Ruth McMahon) wrote:

> Hello,
> I would like to determine whether my protein undergoes phosphorylation,
> preferably by using phospho serine and threonine specific antibodies. If
> anyone has experience using commercial phospho antibodies, I would
> appreciate your recommendations.
> 
> Ruth.
> 
> --
> 
> 
> 
> 
> Ruth McMahon, Ph.D.,                                Tel: 353-1-706-2815
> Biotechnology Centre,                               Fax: 353-1-269-2016
> University College Dublin,
> Belfield,
> Dublin 4,
> Ireland.
> 
> 
> ---
I use Sigma abs against p-Ser and threonine. I was not happy with those at
all. Eventually resorted to orthophosphate labelling in presence/absence
of serine/threonine phosphatase inhibitors like okadaic acid and calyculin
A.

Sourav






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