Growing bugs for plasmid preps

Tom Knight tk at pasteur.ai.mit.edu
Thu Jun 8 12:46:42 EST 2000


We, too, have had difficulty in growing bugs in deep well plates.  You might
want to look at the Stanford web site
http://sequence-www.stanford.edu/group/techdev/shaker.html
which talks about their solution -- commercially available from Genemachines.
The key ideas apparently is to produce turbulence in the well, done with
the correctcombination of speed and throw.  I have no first hand experience
with this system, but I think they are being very successful.






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