Homology Search

[Andrea Hansen]

"Mehdi Alimadadi" <mehdia at interchange.ubc.ca> writes:
> - Please advise me which website I should go for a homology search of a gene
> or a cDNA of a gene in two different species (human and mouse)?
You could use BLAST
http://www.ncbi.nlm.nih.gov/BLAST/
with specialized BLAST pages for special genomes.

> - What is the best way to find a correct and reliable sequence for a gene or
> a cDNA of a gene?
> - Is there any free software to find out the best part of a protein for
> making antibody (epitope), some thing like "MacVector" or "Protean".  I need
> some thing which works with a PC preferably if there is any.  If not I can
> make do with a free program for a Mac!!!!
> - Is there any free software with which you can design primer pairs for a
> gene of interest?

Look at this site:
http://www.bioinformatik.de/cgi-bin/browse/Catalog/PCR/Primer_Design/


Andrea
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