Bacterial RNA isolation

Susanne Rohrer "srohrer(removethis)" at
Tue Jun 13 04:15:16 EST 2000

"M.D. Farrar" wrote:

> Dear all
> Does anyone have a protocol for the efficient extraction of total RNA from
> Staphylococci (or other Gram-positive bacteria)? Our problems are mainly
> associated with insufficient cell lysis and RNA degradation during the
> extraction procedure.

Cheung, AL et al (1994) Anal. Biochem. 222, 511-14

We do use TriZol which he did not like, and the bead-beater is a great little
helper. We have switched to dissolving and storing the RNA in Formamide to
keep it more stable. Good luck!

Susanne Rohrer
Institute of Medical Microbiology
University of Zurich

srohrer at immv dot unizh dot ch

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