ed at molbiol.uct.ac.za
Tue Jun 13 09:32:12 EST 2000
> concern. The problem is that although my results have been consistent
> between replicates, DNA-DNA hybridisation values with homologous samples has
> been consistently lower than when using a heterologous probe. Every possible
> factor that could influence this result has been scrutinized and adapted
> accordingly. We appeal to anyone who could offer us further insight or
> explanation into any areas in this technique that may have been overlooked.
It's just possible that what you are looking at is an artifact of
different numbers of ribosomal RNA genes in different species, or of
highly repeated sequences in the heterologous sequences relative to the
homologous. Other than that, sorry...!
Ed Rybicki, PhD
Dept Microbiology | ed at molbiol.uct.ac.za
University of Cape Town | rybicki at ucthpx.uct.ac.za
Private Bag, Rondebosch | phone: x27-21-650-3265
7701, South Africa | fax: x27-21-689 7573
WWW URL: http://www.uct.ac.za/microbiology/ed.html
"Organised people just have limited horizons"
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