Human SV40 LTAg cell lines
Ian A. York
iayork at panix.com
Tue Jun 13 15:39:55 EST 2000
I want to look at the effects of overexpressing a gene in some human cell
types. My plasmids are all SV40-ori-based. I've seen an effect in COS7
cells (monkey, and therefore specific reagents are in short supply), and I
have seen no effect in 293T cells.
So what I'd like is at least one (and preferably several) human cell lines
which express SV40 large T antigen, and which thereby amplify
SV40-origin-containing plasmids. What's out there that's easily
available?
Ian
--
Ian York (iayork at panix.com) <http://www.panix.com/~iayork/>
"-but as he was a York, I am rather inclined to suppose him a
very respectable Man." -Jane Austen, The History of England
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