isopropranol ppt-n: white precipitate

Student mucineer at iname.com
Wed Jun 14 06:41:00 EST 2000


I have recently done an isopropanol precipitation of plasmid DNA on a
solution eluted from a Qiagen Maxiprep column. On the addition of the
isopropanol, a white cloudy precipitate formed which floated as a layer on
top. Does anyone know what that might be?

I was told that DNA precipitated from isopropanol would look quite glassy
and I shouldn't be able to see it. So could the cloudy layer be salts?
carbohydrate? etc?

Thanks for any insight.









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