Pierce "gentle elution buffer"

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Wed Jun 14 08:53:21 EST 2000


In article <39470B7D.BC613F10 at ludwig.edu.au>, Roger Murphy <
roger.murphy at ludwig.edu.au> wrote:
>A question for the assembled throng....
>
>We're going to use the Pierce "gentle elution buffer" to elute
an
>acid-sensitive monoclonal antibody from a protein A column. 
The Pierce
>instructions warn of dire precipitation consequences if this
buffer ever
>comes into contact with phosphate salts...
>
>Does anyone know why?  What is the ingredient in their buffer
that will
>precipitate with phosphates?
>
>Thanks for your anticipated help!
>
>Roger

ISTR that we talked about this last year or so. Some people
(well,
me at least) speculate that it might be Mg++, which is mildy
chaotropic at high concentrations and forms an insoluble ppt.
with
phosphate.


Nick Theodorakis

nicholas_theodorakis at urmc.rochester.edu
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