PCR from genomic dna

Elizabeth Osterndorff ElizabethO at mail.utexas.edu
Wed Jun 14 08:57:56 EST 2000


> In article <392EE3F3.9C4C5096 at tribe.ulm.edu>,
>   vaidyaks at tribe.ulm.edu (Kedar Vaidya) wrote:
> > I have been trying to get a specific part of the dna amplifies by
> > PCR..the primers seem pretty ok. they work when i pcr from plasmids but
> > i get no product from the genomic dna...any suggestions?

Is it possible that your plasmid contains cDNA, not genomic DNA?  If the area
you're trying to amplify contains a large intron in the genomic sequence then
the pcr will probably fail.

Elizabeth


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