lacZ minus pET expression strain?

dalex at nexus.microimm.mcgill.ca dalex at nexus.microimm.mcgill.ca
Wed Jun 14 14:07:44 EST 2000


Hello All,

I am in search of a lacZ minus strain of E. coli
that is a lysogen for the DE3/T7 polymerase system
(and therefore can be used for pET expression)

The companies I've contacted don't have what I need.
I know it is possible to transduce either the polymerase
or a B-galactosidase deletion,
but if someone out there has already constructed 
such a strain, it would save me much trouble.

Thanks,

David Alexander

dalex at microimm.mcgill.ca

--------------------------------

Department of Microbiology and Immunology
McGill University.
3775 University Street
Montreal Quebec Canada
H3A 2B4


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